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Sterility:

Article Title: Amoxicillin-Resistant Streptococci Carriage in the Mouths of Children: A Systematic Review and Meta-Analysis
Article Snippet: Ready et al. (2004) [ ] United Kingdom 1:S; 2:S; 3:N; 4:S; 5:S; 6:S; 7:S; Score: 6 , A total of 40 children. Group 1 consisted of 25 who had not used any class of ATB in the 3 months prior to sampling, and group 2 consisted of 15 who had used AMPC in the previous 3 months. , Supragingival dental biofilm removed from tooth surfaces. Calcium alginate swab. Iso-Sensitest with 5% defibrinated horse blood used for the isolation. Identification by using molecular biology techniques. , RVC: ≥8 μg/mL. All the children harbored AMPC-resistant bacteria. Of 224 isolates (MIC range, 8 to 128 μg/mL), 128 AMPC-resistant bacteria were isolated from group 1, and 96 isolated from group 2. The median percentage of the total cultivable oral microbiota resistant to AMPC (mainly Haemophilus spp., Streptococcus spp. and Veillonella spp.) was 2.4% in children without AMPC use and 10.9% in children with AMPC use. , The work does not present this data. However, it points out that the 40 (100%) children carried different strains of bacteria resistant to different ATBs.. .. Rotimi et al. (2005) [ ] Kuwait 1:S; 2:S; 3:N; 4:S; 5:N; 6:S; 7:S; Score: 5 , A total of 102 children between 5 and 12 years old of which 88 were from Kuwait and 14 were from other countries. , Supragingival biofilm of deciduous and permanent molars beyond the tongue’s surface. Sterile curettes. Mitis Salivarius agar used for the isolation. Identification by using API 20 Strep test kits (BioMerieux). , RVC: >0.25 μg/mL. In total, 540 strains were isolated. S. salivarius was found most frequently (21.5%), followed by S. sanguis (16.3%). S. mitis (55.9%), S. oralis (55.0%), S. intermedius (52.3%), S. anginosus (45.0%), S. sanguinis (45.2%), S. bovis (41.7%), S. salivarius (39.6%) and S. mutans (36.8%) showed high resistance to AMPC. , The work does not present this data.. .. Salako et al. (2007) [ ] Kuwait 1:S; 2:S; 3:N; 4:S; 5:N; 6:S; 7: S; Score: 5 , A total of 102 healthy and 102 mentally disabled children between 5 and 12 years old. , Supragingival dental biofilm of all primary and permanent molars. Sterile curettes. Mitis Salivarius agar used for the isolation. Identification by using API 20 Strep test kits (BioMerieux). , RVC: >0.25 μg/mL. In total, 741 strains, 330 from healthy children and 411 from those with disabilities. A higher prevalence of S. salivarius in healthy individuals (27.3%) and S. sanguis in disabled individuals (22.6%) was found. A high frequency of AMPC-RS, except S. mutans , was observed in 18% of healthy children and 21% of children with disabilities. , The work does not present this data..

Article Title: Amoxicillin-Resistant Streptococci Carriage in the Mouths of Children: A Systematic Review and Meta-Analysis
Article Snippet: Rotimi et al. (2005) [ ] Kuwait 1:S; 2:S; 3:N; 4:S; 5:N; 6:S; 7:S; Score: 5 , A total of 102 children between 5 and 12 years old of which 88 were from Kuwait and 14 were from other countries. , Supragingival biofilm of deciduous and permanent molars beyond the tongue’s surface. Sterile curettes. Mitis Salivarius agar used for the isolation. Identification by using API 20 Strep test kits (BioMerieux). , RVC: >0.25 μg/mL. In total, 540 strains were isolated. S. salivarius was found most frequently (21.5%), followed by S. sanguis (16.3%). S. mitis (55.9%), S. oralis (55.0%), S. intermedius (52.3%), S. anginosus (45.0%), S. sanguinis (45.2%), S. bovis (41.7%), S. salivarius (39.6%) and S. mutans (36.8%) showed high resistance to AMPC. , The work does not present this data.. .. Salako et al. (2007) [ ] Kuwait 1:S; 2:S; 3:N; 4:S; 5:N; 6:S; 7: S; Score: 5 , A total of 102 healthy and 102 mentally disabled children between 5 and 12 years old. , Supragingival dental biofilm of all primary and permanent molars. Sterile curettes. Mitis Salivarius agar used for the isolation. Identification by using API 20 Strep test kits (BioMerieux). , RVC: >0.25 μg/mL. In total, 741 strains, 330 from healthy children and 411 from those with disabilities. A higher prevalence of S. salivarius in healthy individuals (27.3%) and S. sanguis in disabled individuals (22.6%) was found. A high frequency of AMPC-RS, except S. mutans , was observed in 18% of healthy children and 21% of children with disabilities. , The work does not present this data.. .. Nemoto et al. (2011) [ ] Japan 1:S; 2:S; 3:N; 4:S; 5:S; 6:S; 7:S; Score: 6 , A total of 253 systemically healthy children, teenagers and young adults (2–22 years). , Supra and subgingival biofilm were collected from all teeth. Sterile instrument. Mitis Salivarius agar used for the isolation. Identification by using molecular biology techniques. , RVC: ≥8 μg/mL. Resistant bacteria were selected with selective streptococcal agar with 32 μg/mL of AMPC. In total, 344 strains were isolated; 18 streptococcal strains from 14 patients were highly resistant to AMPC. MICs for these strains ranged from 16 to 64 μg/mL. , A total of 14 children aged between 3 and 9 years..

Isolation:

Article Title: Amoxicillin-Resistant Streptococci Carriage in the Mouths of Children: A Systematic Review and Meta-Analysis
Article Snippet: Ready et al. (2004) [ ] United Kingdom 1:S; 2:S; 3:N; 4:S; 5:S; 6:S; 7:S; Score: 6 , A total of 40 children. Group 1 consisted of 25 who had not used any class of ATB in the 3 months prior to sampling, and group 2 consisted of 15 who had used AMPC in the previous 3 months. , Supragingival dental biofilm removed from tooth surfaces. Calcium alginate swab. Iso-Sensitest with 5% defibrinated horse blood used for the isolation. Identification by using molecular biology techniques. , RVC: ≥8 μg/mL. All the children harbored AMPC-resistant bacteria. Of 224 isolates (MIC range, 8 to 128 μg/mL), 128 AMPC-resistant bacteria were isolated from group 1, and 96 isolated from group 2. The median percentage of the total cultivable oral microbiota resistant to AMPC (mainly Haemophilus spp., Streptococcus spp. and Veillonella spp.) was 2.4% in children without AMPC use and 10.9% in children with AMPC use. , The work does not present this data. However, it points out that the 40 (100%) children carried different strains of bacteria resistant to different ATBs.. .. Rotimi et al. (2005) [ ] Kuwait 1:S; 2:S; 3:N; 4:S; 5:N; 6:S; 7:S; Score: 5 , A total of 102 children between 5 and 12 years old of which 88 were from Kuwait and 14 were from other countries. , Supragingival biofilm of deciduous and permanent molars beyond the tongue’s surface. Sterile curettes. Mitis Salivarius agar used for the isolation. Identification by using API 20 Strep test kits (BioMerieux). , RVC: >0.25 μg/mL. In total, 540 strains were isolated. S. salivarius was found most frequently (21.5%), followed by S. sanguis (16.3%). S. mitis (55.9%), S. oralis (55.0%), S. intermedius (52.3%), S. anginosus (45.0%), S. sanguinis (45.2%), S. bovis (41.7%), S. salivarius (39.6%) and S. mutans (36.8%) showed high resistance to AMPC. , The work does not present this data.. .. Salako et al. (2007) [ ] Kuwait 1:S; 2:S; 3:N; 4:S; 5:N; 6:S; 7: S; Score: 5 , A total of 102 healthy and 102 mentally disabled children between 5 and 12 years old. , Supragingival dental biofilm of all primary and permanent molars. Sterile curettes. Mitis Salivarius agar used for the isolation. Identification by using API 20 Strep test kits (BioMerieux). , RVC: >0.25 μg/mL. In total, 741 strains, 330 from healthy children and 411 from those with disabilities. A higher prevalence of S. salivarius in healthy individuals (27.3%) and S. sanguis in disabled individuals (22.6%) was found. A high frequency of AMPC-RS, except S. mutans , was observed in 18% of healthy children and 21% of children with disabilities. , The work does not present this data..

Article Title: Amoxicillin-Resistant Streptococci Carriage in the Mouths of Children: A Systematic Review and Meta-Analysis
Article Snippet: Rotimi et al. (2005) [ ] Kuwait 1:S; 2:S; 3:N; 4:S; 5:N; 6:S; 7:S; Score: 5 , A total of 102 children between 5 and 12 years old of which 88 were from Kuwait and 14 were from other countries. , Supragingival biofilm of deciduous and permanent molars beyond the tongue’s surface. Sterile curettes. Mitis Salivarius agar used for the isolation. Identification by using API 20 Strep test kits (BioMerieux). , RVC: >0.25 μg/mL. In total, 540 strains were isolated. S. salivarius was found most frequently (21.5%), followed by S. sanguis (16.3%). S. mitis (55.9%), S. oralis (55.0%), S. intermedius (52.3%), S. anginosus (45.0%), S. sanguinis (45.2%), S. bovis (41.7%), S. salivarius (39.6%) and S. mutans (36.8%) showed high resistance to AMPC. , The work does not present this data.. .. Salako et al. (2007) [ ] Kuwait 1:S; 2:S; 3:N; 4:S; 5:N; 6:S; 7: S; Score: 5 , A total of 102 healthy and 102 mentally disabled children between 5 and 12 years old. , Supragingival dental biofilm of all primary and permanent molars. Sterile curettes. Mitis Salivarius agar used for the isolation. Identification by using API 20 Strep test kits (BioMerieux). , RVC: >0.25 μg/mL. In total, 741 strains, 330 from healthy children and 411 from those with disabilities. A higher prevalence of S. salivarius in healthy individuals (27.3%) and S. sanguis in disabled individuals (22.6%) was found. A high frequency of AMPC-RS, except S. mutans , was observed in 18% of healthy children and 21% of children with disabilities. , The work does not present this data.. .. Nemoto et al. (2011) [ ] Japan 1:S; 2:S; 3:N; 4:S; 5:S; 6:S; 7:S; Score: 6 , A total of 253 systemically healthy children, teenagers and young adults (2–22 years). , Supra and subgingival biofilm were collected from all teeth. Sterile instrument. Mitis Salivarius agar used for the isolation. Identification by using molecular biology techniques. , RVC: ≥8 μg/mL. Resistant bacteria were selected with selective streptococcal agar with 32 μg/mL of AMPC. In total, 344 strains were isolated; 18 streptococcal strains from 14 patients were highly resistant to AMPC. MICs for these strains ranged from 16 to 64 μg/mL. , A total of 14 children aged between 3 and 9 years..

Staining:

Article Title: High frequency of isolation of antibiotic-resistant oral Viridans streptococci from children in Kuwait.
Article Snippet: High Frequency of Isolation of Antibiotic-Resistant Oral Viridans Streptococci from Children in Kuwait V.O.. Rotimi, N.O.. Salako, E. Mokaddas, L. Philip & P. Rajan To cite this article: V.O.



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